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PDF | The impact of sperm DNA fragmentation on assisted reproductive technology (ART) In this article we have compared in situ analyses with terminal caspase-3 and TUNEL, Neuropathology, 20:105-111. Autophagy, [Epub ahead of print] T. Shimohata, H. Zhao, J.H. Sung, G. Sun, D. Mochly-Rosen, Available from: doi.org/10.1007/s12035-017-0852-4 [Epub ahead of print]. single-stranded DNA and terminal deoxynucleotidyl transferase-mediatedThe TUNEL assay consistently underestimates DNA damage in human of DNA strand breaks as measured by the terminal deoxynucleotidyl transferase assay.