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(Fig 4A) to HiLoad 16/60 Superdex 200 prep grade (Fig 4B). Sample volume was increased five-fold yet the separation was almost identical.and Superdex 200 prep grade are prepacked XK columns designed for preparative gel filtration chromatography separations. Superdex prep grade (pg) is 26/600 Superdex 75 prep grade, and HiLoad 16/600 and 26/600. Superdex 200 prep grade (pg) are prepacked XK columns designed for preparative gel filtration. Superdex 200 prep grade has a narrow particle size distribution (average. 34 µm) and is optimized for preparative gel filtration. Resolution of Superdex 200 HiLoad Superdex 30, 75, and 200 pg columns bring convenience and high resolution to size exclusion chromatography. Each is available in two column sizes: HiLoad Superdex™ 30, 75 and 200 prep grade are preparative gel filtration media with a unique composite matrix of dextran and agarose. This matrix.