Circoviruses such as PCV are the smallest viruses known and are not expected to be effectively removed by currently-used virus filters due to the small size of the circovirus particles. Anion exchange chromatography such as Q Sepharose® Fast Flow (QSFF) has been shown to effectively remove a range of viruses including parvoviruses. lgG Sepharose ® 6 Fast Flow, from Cytiva, is based on the rigid Sepharose ® 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein. lgG Sepharose ® 6 Fast Flow, from Cytiva, is based on the rigid Sepharose ® 6 Fast Flow matrix, with human IgG covalently coupled to it. Ion Exchange Chromatography - Principles and Methods gelifesciences.com GE, GE monogram, Amersham, ÄKTA, AxiChrom, Biacore, BioProcess, Capto, Cy, CyDye, ECL An axial column (Hitrap Q 5 ml, 2.5 × 1.6 cm) and a radial flow column (3.5 × 5 cm) packed with Q Sepharose Fast Flow media had been evaluated for the separation of human prothrombin. Nitschmann fraction III dissolved in buffered saline (0.10 M sodium chloride buffered with 0.06 M Tris/HCl to pH 7.5) was the starting material. Effects of sample flow rate of the two columns were screened CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications. Manufacturer: Cytiva 17072901 View more versions of this product Catalog No. 45-002-933 $662.50 / Each Qty Check Availability Anion exchange chromatography such as Q Sepharose (®) Fast Flow (QSFF) has been shown to effectively remove a range of viruses including parvoviruses. In this study, we investigated PCV1 removal by SP Sepharose™ Fast Flow, 25 ml 17-0729-10 Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Move containers from fire area if this can be done without risk. Use water spray to keep fire-exposed containers cool. Q Sepharose® Fast Flow is part of the Sepharose® Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with quaternary ammonium (Q) strong anion exchange groups. 33 Accesses Metrics Abstract Q-Sepharose Fast Flow (Q-Sepharose F. F.) was adopted to purify the suspensions of type I, II, III oral poliovaccine (OPV), which is prepared from Vero cells. After clarification and concentration by ultrafiltration, the recovery of virus infected titre may attain above 85%. Initial Q Sepharose Fast Flow Column"& Sepharose fast flow resin (QSFF, Pharmacia Biotech Inc.) was pre-equilibrated in SLB. The col- umns were poured in 2.5 x 20-cm Bio-Rad Econo-Columns. A 50-ml column was used for every 450-550 ml of crude supernatant. The sample was applied at a flow rate of 5-9 mumin (6-10 midcolumn volume). Use for preparative protein separations in both research and industrial applications. Cytiva Q Sepharose™ Fast Flow Chromatography Media is a strong anion exchanger based on the well established Sepharose Fast Flow ion exchange platform.