qiaquick pcr purification kit (50)pcr purification principle
qiaquick pcr purification kit (250) protocol
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qiaquick pcr purification kit (250)

This protocol is for the purification of up to 10 µg PCR products (100 bp to 10 kb in size). ○ Add ethanol (96–100%) to Buffer PE before use (see bottle Use this protocol for purifying PCR products only. If purifying fluorescence-labeled cDNA from uncoupled or unincorporated fluorescent dye, see Appendix. 1. AddThis protocol is designed to purify single- or double-stranded DNA fragments from PCR. Fragments ranging from 100 bp to 10 kb are purified from primers,. Transfer PCR product (not including oil) to 1.5ml microcentrifuge tube. 2. Add 5 volume PCR Purification buffer and mix by tapping. Remove impurities by thorough washing with Wash Buffer. To purify the. DNA, elute the dsDNA in low salt Elution Buffer or water. The purified PCR product is The kit is designed for the efficient and convenient isolation of PCR products from amplification reactions. Primers,. PCR products can be sequenced directly using cycle sequencing methods, as long as there is only one product present and the primers and excess dNTP's. QIAquick PCR Purification Kit Protocol using a microcentrifuge. This protocol is designed to purify single- or double-stranded DNA fragments from PCR.