1.3. Types of cell cultures. Broadly, animal tissue culture can be divided into two categories: Cultures that allow cell-cell interactions and encourage communication or signaling between cells. Cultures in which cell-cell communication or interactions are lost or the signaling between them is missing. Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via the use of a liquid, semi-solid, or solid growth medium, such as broth or agar, in vitro under sterile growing conditions. Banana is typically propagated vegetatively; thus tissue culture as a propagation technique provides a robust 11. 11 Basic requirements of Plant Tissue Culture: 11 •Plant material •Equipments and Glasswares •Aseptic Condition •Washing and storage facilities •Media preparation room •Sterilization room •Nutrient medium •Transfer room •Culture room or incubators •Proper and optimum aeration •Well equipped observation or recording area Uses of Plant tissue culture. Tissue culture is used to develop thousands of genetically identical plants from one single parent plant known as somaclones, and this process is known as micropropagation. The method offers an advantage over other methods as it can be used to develop disease free plants from disease-rode plants by using their Most plant tissue cultures are incubated in reach-in growth chambers or growth rooms. Growth facilities should provide temperature control, variable light intensity of appropriate wavelengths, photoperiod control, and in some cases humidity control (Gamborg and Phillips 1995 ). Temperatures should be controlled to ± 1°C. Tissue culture, also known as micropropagation, is a propagation method used to produce plants under sterile conditions. This method uses plant explants (plant parts) or seeds that have been sterilized before being placed in containers with a growing medium (usually a gel) that has some nutrients added. The explants or seeds, the containers ADVERTISEMENTS: Let us make an in-depth study of the definition, principle, protocol and importance of the cell suspension culture. Definition: Suspension culture is a type of culture in which single cells or small aggregates of cells mul­tiply while suspended in agitated liquid medium. It is also referred to as cell culture or cell suspen­sion culture. […] Tissue culture involves the in vitro maintenance and propagation of cells in optimal conditions. Culturing animal cells, tissue or organs in a controlled artificial environ-ment is called animal tissue culture. The importance of animal tissue culture was initially realized during the development of the polio vaccine using primary monkey emergencies, nearly all tissue types can return to the meris-tematic state, and divide forming replacement tissues needed to repair wounds, broken and damaged shoots and roots. This fast dividing replacement tissue called callus can be induced in various in vitro-cultured explants by external ad-dition of plant growth regulators (PGRs). the ability of the plant tissues to survive severe tissue water loss. A number of elegant experi ments have used tissue culture to study metabo lism in resurrection plants. The time is now ripe for the large-scale molecular analysis of the poikilohydric ability of these plants using tissue culture as a major tool to drive research forward 2.1 Plant Growth P