The stain was originally developed by Christian Gram in 1884 (4). The modification currently used for general bacteriology was developed by Hucker in 1921, providing improved consistency of decolorization and better differentiation of organisms (5). gram's stain; Issue Section: Articles. Download all slides. Advertisement. Citations. Views. 176,930. Altmetric. This PDF is available to Subscribers Only. View Article Abstract & Purchase Options. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. ABSTRACT Gram staining is a rapid, easily learned laboratory technique. Although commonly used in other clinical areas, Gram staining has not been in standard nurse-midwifery practice. View the article PDF and any associated supplements and figures for a period of 48 hours. Article can not be printed. Article can not be downloaded. Here we propose the use of Gram staining as a fast and cost-effective diagnostic tool to differentiate the previously mentioned differential diagnoses, especially in resource-limited areas. If Gram staining is performed, the usual findings compatible with S. aureus would yield Gram-positive cocci in clusters [2]. PDF Tools Share Abstract Named after Hans Christian Gram who developed the method in 1884, the Gram stain allows one to distinguish between Gram-positive and Gram-negative bacteria on the basis of differential staining with a crystal violet-iodine complex and a safranin counterstain. Share this article. Anyone you share the following link with will be able to read this content: Get shareable link The Gram staining method, the most important staining procedure in Microbiology, was developed by Danish physician Hans Christian Gram in 1884. Gram staining is still the cornerstone of bacterial identification and taxonomic division. This differential staining procedure separates most bacteria into two groups on the basis of cell wall composition; Procedure of Gram Staining. Take a clean, grease free slide. Prepare the smear of suspension on the clean slide with a loopful of sample. Crystal Violet was poured and kept for about 30 seconds to 1 minutes and rinse with water. Flood the gram's iodine for 1 minute and wash with water. Then ,wash with 95% alcohol or acetone for about 10-20 Gram staining is a differential bacterial staining technique used to differentiate bacteria into Gram Positive and Gram Negative types according to their cell wall composition. It is the most widely used and the most important staining technique in bacteriology, especially in medical bacteriology. Gram staining -Requirements •Gram-staining is a four part procedure. •The specimen is mounted and heat fixed on a slide before proceeding to stain it. •The reagents required are: Crystal Violet (the Primary Stain) Iodine Solution (the Mordant) Decolorizer (ethanol) Safranin (the Counter stain) Water (preferably in a squirt bottle) Reagents of Gram Stain Because the Gram stain reaction is more widely used and studied than any other stain, it is also the most open to variation in protocol. Four reagents are used in the Gram procedure: 1. A primary stain--originally aniline-gentian violet was used, but this has been replaced by the more stable crystal violet. 2. 4. Finally, a counter stain (Safranin), is applied to stain those cells (Gram Negative) that have lost the primary stain as a result of decolorization Gram Reaction Gram-positive bacteria are those that are stained dark blue