crispr assisted editing of bacterial genomes
multigene editing in the escherichia coli genome via the crispr-cas9 system
pcas9 marraffinigenome engineering using the crispr cas9 system

1 Mar 2013 Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. We reprogram dual-RNA:Cas9 specificity by changing the sequence of short CRISPR RNA (crRNA) to make single- and multinucleotide changes carried on editing templates. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Experiment 1. Null hypothesis: When the genome contains the appropriate target DNA, This is one of several recent studies (see also {1-3}) describing how the emerging new properties of this defense system are being repurposed for genome RNA-guided editing of bacterial genomes using CRISPR-Cas systems (English) . CRISPR RNA maturation by trans-encoded small RNA and host factor Articles RNA-guided editing of bacterial genomes using CRISPR-Cas systems Wenyan Jiang1,4, David Bikard1,4, David Cox2,3, Feng Zhang2,3 & Luciano ARequest PDF on ResearchGate | RNA-guided editing of bacterial genomes using CRISPR-Cas systems | Here we use the clustered, regularly interspaced, short A CRISPR-Cas system is harnessed to introduce template-driven mutations in S. pneumoniae and E. coli at high e | Wenyan Jiang, David Bikard, David RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA Nat Biotechnol. 2013 Jan 29. doi: