0.1 ml Bioruptor® Microtubes for Bioruptor® Pico bag of 120. Tel: No manual is currently available for this item. Click here to request more details. Help / Quotation Request Form. Please fill in the boxes below to request help from a technical specialist. The IP-Star® Compact replaces the numerous manual, error-prone steps of complex epigenetic applications with a reliable, highly consistent and automated process that requires minimal operator intervention. In addition, the Bioruptor® Pico MicroPlex Library The Bioruptor NGS is used exclusively for DNA shearing and provides fragments up to 1.2 kb suitable for next gen sequencing. It is located in the lab and connected to a water cooling system, which automatically cycles on between sonication modes. The Bioruptor NGS is covered with a soundproof box, so ear protection is not required. The Bioruptor Pico provides accurate and consistent DNA fragmentation of up to 12 samples simultaneously, and it is capable of shearing samples down to 200 bp in less than 15 min. 7. Prepare 15mL green cap tubes used in Pico Bioruptor by adding 550mg diagenode sonication beads and wash with 1mL PBS and wash with 1mL RIPA. 8. Transfer +1.2mL nuclear lysate (2X10^7 cells) into 1x15ml green cap tubes and keep 1.5mL tube for hard spin after sonication. Sonicate for 20' (30" on, 30" off) on Pico Bioruptor. 9. The Bioruptor® Pico is the latest innovation in shearing and phenol/chloroform purified as described in the kit manual. 10 ?l of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker). The Bioruptor® Plus is specifically designed for ChIP-PCR and it can also be used for DNA methylation analysis. The Bioruptor® Pico (modern evolution of the Bioruptor® NGS) is the most advance system for NGS. The Diagenode One is the new desktop solution that provides optimal DNA shearing. Laboratory Balances 98648-019-79 98648-019-79 Buy Sartorius Balances at www.ba