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I treated the water with it yesterday. However, i have just sat down and read the leaflet enclosed back-to-back and the instructions on the Suitable for tropical freshwater and coldwater aquaria. Directions. Tighten cap & shake bottle. Use the cup to measure the correct dose (see table below). Protozin is our best selling disease treatment world wide. Suitable for tropical freshwater and coldwater aquaria. Directions: Tighten cap & shake bottle. Use