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Minimal signal amplification. 2. Indirect ELISA. For indirect detection, the antigen coated to a multi-well plate is detected in two stages or layers. INDIRECT ELISA ptglab.com. 1. 2. 4. 3. Antigen coating: a. Dilute purified antigens to a final concentration of 3.0 μg/ml in antigen-coating. The indirect ELISA assay is commonly used to measure the amount of antibodies in serum or in the supernatant of a hybridoma culture. The. Indirect ELISA. Figure 3 demonstrates how an indirect ELISA is set up; antigen is adsorbed to a well in an. ELISA plate . Detection is a two-step process . antigens in the sample) ELISA methods are the two most common types used. Page 20. The Indirect ELISA Method – Part 1 a) Binding Known Antigen -Indirect ELISA protocol. Buffers and Reagents: (See Direct Elisa protocol buffers and reagents). For accurate quantitative results, always compare signal of
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