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Biological indicators, or spore tests, are the most accepted means of monitoring sterilization because they assess the sterilization process directly by killing No growth. No growth. Indicator may be expired; test again with unexpired biological indicators. Page 2. 5/2016. 2. BIOLOGICAL INDICATOR TEST INSTRUCTIONS. Monitoring Effectiveness of Sterilization of Common Biology Autoclaves (instructions are those for NAMSA's SCBIs noted above – if using a different 7. Firmly seal the Verify® BI by following the recommended technique shown in figures 1 and 2 of the manufacturer's instruction pamphlet. The indicator cap.Autoclaves used to treat biological waste will be evaluated with a biological indicator by EHS on a quarterly basis. Autoclave the load along with the indicator following the 'Autoclave Manual' instructions. When the autoclave cycle ends, remove the indicator vial from the