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PRINCIPLE: Agarose gel electrophoresis used to analyze and quantitate nucleic acid. The. Agarose for Agarose gel electrophoresis is purified from agar. At the end of the experiment the gel has various bands of separated substances. Page 2. When DNA is the substance being separated it must first be cut up into What would happen to the DNA if you used a 2% agarose gel, but ran it for the same amount of time? 2. (a) Based on these results, which step of the experiment Develop important skills in gel electrophoresis, a widely used technique for the preparation and analysis of DNA and proteins. Prerequisite Skills: • Prior Electrophoresis Buffer – contains ions necessary to conduct an electrical current, maintains pH of experiment. • Horizontal electrophoresis apparatus – holdsAgarose gel electrophoresis is a widely used procedure in various areas of biotechnology. This simple, but precise, analytical procedure is used in. Introduction: Agarose gel electrophoresis is method for separation (by size), quantifying, purification of nucleic acids fragments mixture, and analysis of Agarose gel electrophoresis is a simple, cheap and highly effeccve method for separacng, idencfying, and purifying DNA fragments. • We will use agarose gel