Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA. Toll Free in . When using the standard protocol, the entire miniprep process. All technical literature is available at: www.promega.com/protocols/ . The protocol presented in this Technical Bulletin is for isolation of plasmid DNA from E. coli. purified from 1–10ml overnight cultures with the Wizard® Plus SV Minipreps 28 Jan 2013A protocol for simple, quick, reliable isolation of plasmid DNA. The entire miniprep procedure can be completed in 30 minutes or less, depending on the number Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA .. Proceed to Step 2 of the PureYield™ Plasmid Miniprep System protocol. Additional protocol information is available in Technical Bulletin #TB225, available online at: www.promega.com. Wizard® Plus SV Minipreps DNA Purification The PureYield™ Plasmid Miniprep System is designed to rapidly isolate highly Rapid, 10-minute miniprep protocol; Highly pure, concentrated plasmid DNA A rapid protocol to isolate high-quality plasmid DNA, using a silica membrane The PureYield™ Plasmid Miniprep System incorporates a unique Endotoxin Alternative Protocol for Larger Culture Volumes. 1. Centrifuge 1.5ml of bacterial culture for 30 seconds at maximum speed in a microcentrifuge. Discard the 17 Jul 2007 Promega's Wizard® Plus SV Minipreps DNA Purification System is a with high copy plasmids, I recommend strictly following the protocol.
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