vi Liquid Chromatography-Mass Spectrometry 2.5 Quantitation Using High Performance Liquid Chromatography 23 2.6 The Need for High Performance Liquid Chromatography- Mass Spectrometry 30 References 31 3 Mass Spectrometry 33 3.1 Introduction 33 3.2 Ionization Methods 36 3.2.1 Electron Ionization 36 3.2.2 Chemical Ionization 36 3.2.3 Fast-Atom

Colorless liquid or gas (above 69F) with a pungent, fruity odor. NIOSH AB1925000: colourless liquid Oxford University Chemical Safety Data (No longer updated) More details: flammable, colourless liquiduid/pungent ethereal odour Food and Agriculture Organization of the United Nations Acetaldehyde Spectra for C4H10 58 Mass spectrum for butane 43 29 Mass spectra for Cl2 and Br2 If a molecule is put through a mass spectrometer with Electro Spray Ionisation then fragmentation will not occur. There will be one peak that will equal the mass of the MH+ ion. It will therefore be necessary to subtract 1 to get the Mr of the molecule. So if a One chlorine atom in a compound . The molecular ion peaks (M+ and M+2) each contain one chlorine atom - but the chlorine can be either of the two chlorine isotopes, 35 Cl and 37 Cl. The molecular ion containing the 35 Cl isotope has a relative formula mass of 78. The one containing 37 Cl has a relative formula mass of 80 - hence the two lines at m/z = 78 and m/z = 80. Mass Spectrometry: What is it? Mass spectrometry is an analytical tool that makes use of the mass to charge ratio of particles to determine the molecular formula of a compound. In the lab, this tool can be immensely powerful in making sure what you make is actually what you want to make. The Theory Behind It The mass spectrum of chlorine. Chlorine is taken as typical of elements with more than one atom per molecule. We'll look at its mass spectrum to show the sort of problems involved. Chlorine has two isotopes, 35 Cl and 37 Cl, in the approximate ratio of 3 atoms of 35 Cl to 1 atom of 37 Cl. You might suppose that the mass